Two iQ5 machines are available for use, both located on the. Up to a 1-year Warranty & Lifetime Support. The Core manages 2 BioRad iQ5 real time PCR machines and one digital droplet PCR system QX200. Plexor HY System and provides instructions for use with the Bio-Rad iQ5 Real-Time PCR. Use of iQ SYBR Green Supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude. Refurbished Bio-Rad MyiQ Single-Color Real-Time PCR Detection System, tested & serviced prior to shipping. This supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Bio-Rad IQ5 with: Fluorescence Detection range: 515 nm-700 nm Excitation range: 475 nm-645 nm Dynamic range: 9 orders of magnitude Dimensions: Width: 11.5' Depth: 23' Height: 15.2' Electronics: 100-120/220-240 VAC 60 hz 8/4 amps Location Details: Southwest US This machine is located at a client’s facility in Southwest US. Operating the BioRad iQ5 softwareBefore opening the program. Close the iQconfig application by clicking in the top right corner. You will be asked if you want to replace the existing file. Select the file c:Program FilesBio-RadiCyclerIniFactor96.ini and click Save. This preblended 2x solution contains an optimized concentration of SYBR Green I dye for sensitive and accurate detection, hot-start iTaq DNA Polymerase, a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR Green I, and qPCR-qualified dNTPs. Instruction Manual, iQ5 Optical System Software, Rev B. Pkg of 25, semi-skirted PCR plate for iCycler iQ, iQ5, MyiQ real-time PCR. Real-time PCR detection was performed with a SYBR Green supermix (Bio-Rad, Hercules, CA. The 'Select Target Factor File' dialog box will open. IQ SYBR Green Supermix is formulated to achieve optimal results for a wide array of real-time PCR applications with the convenience of SYBR Green I detection chemistry.
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